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KMID : 0378019730160120071
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New Medical Journal
1973 Volume.16 No. 12 p.71 ~ p.76
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Autoradiography as a Tool of Histochemistry
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南周鉉/Nam, Ju Hyun
金一雲/朱剛/張性翼/李隆昌/Kim, Il Woon/Joo, Kang/Chang, Sung Ik/Lee, Yung Chang
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Abstract
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The techniques by which radioactivity may be detected with photographic emulsios are basically very simple. In theory, radioautography (or autoradiography) is a method by which the silver bromide crystals of photographic emulsions act as microdetectors of radiation. Since the crystals are very small (a fraction of a p), it is theoretically possible to locate radioelements in corresponding small structure of histological sections. The crystals which happen to be close to the radioactive structures in the sections receive the impact of the radiation and, upon development, are transformed into black silver. grains. Although the applications of autoradiography in many fields of biology are very popular, unfortunately, these techniques tend to be described in papers primarilly written on a wide range of biological subjects, appearing in an equally wide spectrum of journals. Furthermore, eath paper often only describes one technque, and it is difficult to find adequate discussion of the relative merits of different techniques, and their applicalbility to various types of research.
The aim of this paper is, therefore, to describe the principles underlying the detection of radioactivity
by autoradiography, and to discuss the various techniques at present available to the biology.
The advantages and disadvantages of each method are considered so that the most suitable approach
to any particular research problem can be determined.
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